This take on the classic comic book franchise mixes familiar characters with new villains not previously seen in animated form. As usual, Batman tries to rid Gotham City of evil, but he's not doing it alone. Assisting the Caped Crusader are retired MI-6 operative Alfred Pennyworth, who also serves as the superhero's butler, and lethal swordswoman Tatsu "Katana" Yamashiro. Among the villains who lead Gotham City's criminal underworld that Batman and his crew battle are Anarky, Professor Pyg, Mister Toad and Magpie.